Microinjection robot

For injection of compounds, protein, DNA, gRNA, etc. in the chorion, yolk or first cell of zebrafish eggs.

Quick facts

Suitable for zebrafish eggs

Air-pressure injection

Deep learning for automatic detection of injection position

On-site training included

Up to 1000 eggs in 25 minutes

Protocol

Using a mould a grid of wells is formed in agarose, in a special grid holder plate (included and reusable). There are multiple choices of moulds available, depending on your application. E.g. 1024 wells, 9 times 100 wells, 60 times 7 wells, etc.
Eggs are collected, and are placed into the wells using a small brush (3-5 min)
The glass pulled needle is filled and the plate is placed in the robot.
The user can select the injection program to inject part of( eg 100 eggs) or the whole plate (eg 1000 eggs) automatically in the chorion, yolk or first cell*.
Injection speed is around 1000 eggs in 25 minutes.

Customization

It is possible to inject in other organisms or samples, please ask about the possibilities for your application of interest.

Examples

The video below shows microinjections in zebrafish eggs, where the injection position is determined by deep-learning software.

Pricing and ordering

Based on your request and application there are different options available that will affect the price. Therefore, pricing information is only available on request. Academic discount is available, and the purchase includes on-site installation and two days of training. Commercial use requires a yearly license and service contract.

For ordering or more information, please contact us here.

Publications

2024

Comparing robotic and manual injection methods in zebrafish embryos for high-throughput RNA silencing using CRISPR-RfxCas13dJoaquin Abugattas-Nuñez Del Prado, Yi Ding, Jan de Sonneville, Kees-Jan van der Kolk, Miguel A Moreno-Mateos, Edward Málaga-Trillo and Herman P SpainkBioTechniques(open access)

2021

Anti-tuberculosis Compound Screen using a Zebrafish Infection Model identifies an Aspartyl-tRNA Synthetase InhibitorEva Habjan, Vien QT Ho, James Gallant, Gunny Van Stempvoort, Kin Ki Jim, Coen Kuijl, Daan P. Geerke, Wilbert Bitter, Alexander SpeerDisease Models & Mechanisms(open access)

2019

Deep learning image recognition enables efficient genome editing in zebrafish by automated injectionsMaria Lorena Cordero-Maldonado, Simon Perathoner, Kees-Jan van der Kolk, Ralf Boland, Ursula Heins-Marroquin, Herman P. Spaink, Annemarie H. Meijer, Alexander D. Crawford, Jan de SonnevillePLoS ONE(open access)

2015

Testing Tuberculosis Drug Efficacy in a Zebrafish High-Throughput Translational Medicine ScreenAnita Ordas, Robert-Jan Raterink, Fraser Cunningham, Hans J. Jansen, Malgorzata I. Wiweger, Susanne Jong-Raadsen, Sabine Bos, Robert H. Bates, David Barros, Annemarie H. Meijer, Rob J. Vreeken, Lluís Ballell-Pages, Ron P. Dirks, Thomas Hankemeier, Herman P. SpainkAntimicrobial Agents and Chemotherapy(open access)

2014

Establishment and Optimization of a High Throughput Setup to Study Staphylococcus epidermidis and Mycobacterium marinum Infection as a Model for Drug DiscoveryWouter J. Veneman, Rubén Marín-Juez, Jan de Sonneville, Anita Ordas, Susanne Jong-Raadsen, Annemarie H. Meijer, Herman P. SpainkJ. Vis. Exp.(open access)

2013

Robotic injection of zebrafish embryos for high-throughput screening in disease modelsHerman P. Spaink, Chao Cui, Malgorzata I. Wiweger, Hans J. Jansen, Wouter J. Veneman, Rubén Marín-Juez, Jan de Sonneville, Anita Ordas, Vincenzo Torraca, Wietske van der Ent, William P. Leenders, Annemarie H. Meijer, B. Ewa Snaar-Jagalska, Ron P. DirksMethods(open access)

A zebrafish high throughput screening system used for Staphylococcus epidermidisinfection marker discoveryWouter J. Veneman, Oliver W. Stockhammer, Leonie de Boer, Sebastian A.J. Zaat, Annemarie H. Meijer, Herman P. SpainkBMC Genomics(open access)