Automated microinjection system for zebrafish larvae

For injection of foreign substances, such as dye, compounds, bacteria, cancer cells, immune cells, and microspheres, into the duct of Cuvier, perivitelline space, and hindbrain ventricle of 2-day post fertilization (dpf) zebrafish larvae.

Quick facts

Suitable for dechorionated zebrafish larvae (1-5 dpf)

Recognition of zebrafish larvae and injection sites

Automatic needle rotation to fit the orientation of larvae

Automatic droplet calibration

No manual alignment of zebrafish larvae needed

10 × magnification showing larvae details

Automatic puncture detection

Fully automated injection

Both automatic and manual modes are available

Touch screen operation

Air-pressure injection

On-site training included

Protocol

Choose the injection settings, such as the development stage of zebrafish larvae, injection site (duct of Cuvier, perivitelline space, and hindbrain ventricle), injection location, and injection macro.
Needle calibration and droplet calibration.
Prepare an agarose gel plate and place anesthetized zebrafish larvae randomly on the plate.
Place the plate and start the injection.

Customization

It is possible to inject in other injection sites and other organisms; please ask about the possibilities for your application of interest.

Examples

Automatic microinjection of dye into the duct of Cuvier of 2 dpf zebrafish larvae

Automatic microinjection of cancer cells into the perivitelline space of 2 dpf zebrafish larvae

Automatic microinjection of dye into the hindbrain ventricle of 2 dpf zebrafish larvae

Pricing and ordering

Based on your request and application there are different options available that will affect the price. Therefore, pricing information is only available on request. Academic discount is available, and the purchase includes on-site installation and two days of training. Commercial use requires a yearly license and service contract.

For ordering or more information, please contact us here.

Publications

2025

Automated microinjection for zebrafish xenograft modelsYi Ding, Kees-Jan van der Kolk, Wietske van der Ent, Michele Scotto di Mase, Saskia Kowald, Jenny Huizing, Jana M. Vidal Teuton, Gunja Mishra, Maxime Kempers, Rusul Almter, Sandra Kunz, Laurine Munier, Carl Koschmann, Sebastian M. Waszak, Vincenzo Di Donato, Sylvia Dyballa, Peter Ten Dijke, Camila Vicencio Esguerra, Lasse D. Jensen, Jan de Sonnevillenpj Biomedical Innovations(open access)