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Microinjection robot for 3D cell cultures
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Micro-injection robot for 3D cell cultures

For injection of small droplets of cells into a hydrogel.

Quick facts

Creation of cell spheroids in a gel matrix
Displacement injection
Programmable injection patterns
On-site training included

Protocol

For this application, cells from culture or tissue are dissociated, washed and filtered into a single cell suspension. The suspension is pipetted into a well of a 96 or 384 well plate. In the same or another plate, wells are pre-filled with e.g. collagen gel and using a set volume and location multiple wells are automatically injected with droplets of cells.

Known applications

Cancer cell spheroid migration assay

Using droplets of about 300 micrometer diameter (±10k cells) cells in the core of the spheroid will die, and act as a trigger to start migration of cells. The droplet diameter as well as the outgrowth is reproducible, making this method suitable for high content screening assays. Typical time needed for one screen is approximately 3 (cell culture cells) to 8 (primary cells) days.

Cell-cell interaction assay

In this application, cell spheroids are placed at a predefined distance of each other in a gel, and the cell-cell interaction can be studied using e.g. confocal microscopy. In addition to the distance also the time between injections can be varied, depending on your application. Using multiple cell types in one well, more complex tissues can be formed and studied.

Pricing and ordering

Based on your request and application there are different options available that will affect the price. Therefore, pricing information is only available on request. Academic discount is available, and the purchase includes on-site installation and two days of training. Commercial use requires a yearly license and service contract.


For ordering or more information, please contact us here.

Publications

2017
NAD Synthesis Pathway Interference Is a Viable Therapeutic Strategy for ChondrosarcomaElisabeth F.P. Peterse, Brendy E.W.M. van den Akker, Bertine Niessen, Jan Oosting, Johnny Suijker, Yvonne de Jong, Erik H.J. Danen, Anne-Marie Cleton-Jansen and Judith V.M.G. BovéeMolecular Cancer Research(open access)
2016
Tumor-induced remote ECM network orientation steers angiogenesisBalcioglu, H., van de Water, B. & Danen, E.Nature Scientific Reports(open access)
2014
SYK is a candidate kinase target for the treatment of advanced prostate cancerVeerander Paul Singh Ghotra, Shuning He, Geertje van der Horst, Steffen Nijhoff, Hans de Bont, Annemarie Lekkerkerker, Richard Janssen, Guido Jenster, Geert JLH van Leenders, Agnes Marije M. Hoogland, Esther I Verhoef, Zuzanna Baranski, Jiangling Xiong, Bob van de Water, Gabri van der Pluijm, Ewa Snaar-Jagalska and Erik DanenCancer Research
β1 Integrin Inhibition Elicits a Prometastatic Switch Through the TGFβ–miR-200–ZEB Network in E-Cadherin–Positive Triple-Negative Breast CancerHoa H. Truong, Jiangling Xiong, Veerander P. S. Ghotra, Ella Nirmala, Lizette Haazen, Sylvia E. Le Dévédec, Hayri E. Balcioğlu, Shuning He, B. Ewa Snaar-Jagalska, Erno Vreugdenhil, John H. N. Meerman, Bob van de Water, Erik H. J. DanenScience Signaling
EphA receptors regulate prostate cancer cell dissemination through Vav2–RhoA mediated cell–cell repulsionJennifer Batson, Lucy Maccarthy-Morrogh, Amy Archer, Helen Tanton, Catherine D. NobesBiology Open(open access)
Reproduced manual injection to generate spheroids
2012
Automated microinjection of cell-polymer suspensions in 3D ECM scaffolds for high-throughput quantitative cancer invasion screensHoa H. Truong and Jan [de Sonneville] and Veerander P.S. Ghotra and Jiangling Xiong and Leo Price and Pancras C.W. Hogendoorn and Herman H. Spaink and Bob [van de Water] and Erik H.J. DanenBiomaterials