zebrafish microinjection (5) - cancer spheroids (5) - GeneTiles (4)
Testing Tuberculosis Drug Efficacy in a Zebrafish High-Throughput Translational Medicine Screen Antimicrobial Agents and Chemotherapy (open access)
Establishment and Optimization of a High Throughput Setup to Study Staphylococcus epidermidis and Mycobacterium marinum Infection as a Model for Drug Discovery JOVE (open access)
2013 - 2011
Robotic injection of zebrafish embryos for high-throughput screening in disease models Methods (open access)
A zebrafish high throughput screening system used for Staphylococcus epidermidis infection marker discovery BMC Genomics (open access)
A High-Throughput Screen for Tuberculosis Progression PLoS ONE (open access)
Tumor-induced remote ECM network orientation steers angiogenesis Nature Scientific Reports (open access)
SYK is a candidate kinase target for the treatment of advanced prostate cancer Cancer Research
Β1 Integrin Inhibition Elicits a Prometastatic Switch Through the TGFΒ–miR-200–ZEB Network in E-Cadherin–Positive Triple-Negative Breast Cancer Science Signaling
EphA receptors regulate prostate cancer cell dissemination through Vav2–RhoA mediated cell–cell repulsion Reproduced manual injection to generate spheroids Biology Open (open access)
2013 - 2011
Automated microinjection of cell-polymer suspensions in 3D ECM scaffolds for high-throughput quantitative cancer invasion screens Biomaterials
Common and specific downstream signaling targets controlled by Tlr2 and Tlr5 innate immune signaling in zebrafish BMC Genomics (open access)
Analysis of RNAseq datasets from a comparative infectious disease zebrafish model using GeneTiles bioinformatics Immunogenetics (open access)
GLUT12 deficiency during early development results in heart failure and a diabetic phenotype in zebrafish Journal of Endocrinology (open access)
Hyperinsulinemia induces insulin resistance and immune suppression via Ptpn6/Shp1 in zebrafish Journal of Endocrinology (open access)
In GeneTiles the visualization and browsing of RNAseq data is greatly improved. Open source alignment and statistics programs required to map raw illumina data-files are integrated in this platform and the whole data analysis pipeline is kept open for scientific inspection and reproducibility. Differential expression of genes is shown in a 2D array of tiles. The tile color and intensity are a measure of the significance of the differential expression. When a tile is selected, the gene is loaded underneath, scaled to fit the width of the screen. In a schematic view all introns are shrunk to a fixed short length to visualize the aligned reads in a graph above the exons.
GeneTiles is published in Immunogenetics, Dec 2014
View GeneTiles demo: www.GeneTiles.com
Limited free trial available, please contact us
Microinjection is used for decades, however this technique has only recently been redesigned to fit the specific needs of modern high-throughput research.
Using a special grid, zebrafish eggs are aligned such that microinjection can take place at an unprecedented speed of 2500 eggs per hour.
The discovery of the possibility to infect and monitor tuberculosis progression in zebrafish is described in this paper. (open access, PLoS ONE, 2010)
More information on screening in zebrafish is available @ ZF-Screens BV
Mixing polymers with a cell suspension allows for instant cell tissue creation at predefined locations in a hydrogel.
The use of these injected cell spheroids in high-throughput quantitative cancer invasion screens is described in this paper. (Biomaterials, 2012)
By automated injection of a cell suspension derived from cell culture or primary tissue material into collagen gel with a glass pulled needle, one can obtain adhered tightly packed cell spheroids within a couple of hours.
Advantages to other methods:
- Spheroid formation compatible with all cell-types
- Reproducible spheroid size (ANOVA, P=1.00) and spheroid outgrowth (ANOVA, P=0.80)
- Cell-cell and cell-matrix contacts are created directly after injection
- Spheroid postions are predetermined, ideal for high-throughput microscopy, compatible with e.g. Opera® High Content Screening System
- Compatible with all gel types and 96, 384 and 1536 titre plates
- Co-cultures possible by using a gel mixed with cells, or by injecting multiple cell-types next to each other in the same gel
Chamber of commerce: 53301978
Development of high-throughput methods for cell biology applications
for both life sciences and medical research.
Consultancy on method optimisation and pipeline development.
Sold the sixth AMS to Camila Vicencio Esguerra (Chemical Neuroscience (BIOCEG), University of Oslo, Norway)
Sold the fifth AMS to Alexander Crawford (Chemical Biology Group, LCSB, University of Luxembourg, Luxembourg) for the application of morpholino injections
Sold the fourth AMS to the Dasman Diabetes Institute in Kuwait.
Sold the third AMS to Astrid van der Sar (MMI research, VUMC, Amsterdam, NL)
Sold the second AMS to Erik Danen (Toxicology, LACDR, Leiden University, NL)
Developed and sold the first Automated Microinjection System (AMS) to Herman Spaink (MCB, Leiden University, NL)
Jan de Sonneville LinkedIn
Life Science Methods BV
JH Oortweg 19
2333 CH Leiden
The Netherlands (google maps)
+31 71 332 2345